Effects of actinomycin D and cycloheximide on the increase in tyrosinase activity of hamster amelanotic

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Abstract
Tyrosinase activity in the Ab hamster amelanotic melanoma cells cultured in serum-free Eagle''s MEM [minimum essential medium] increased 3 times after 6 h of primary cell culture. This increase was inhibited completely by cycloheximide, while actinomycin D had no effect on this process. After 24 h of culture in MEM with calf serum, further increase of the tyrosinase activity was inhibited by both cycloheximide and actinomycin D. The increase of tyrosinase activity in the cell culture of the Ab melanoma evidently is due initially to the unblocking of translation and later to the activation of transcription of the gene controlling the enzyme.