Solid‐phase synthesis of O‐mannosylated peptides: two strategies compared
- 1 August 1993
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 42 (2) , 165-170
- https://doi.org/10.1111/j.1399-3011.1993.tb00493.x
Abstract
A comparison of the O-glycosylation of resin-bound assembled peptides with the incorporation of glycosylated amino acids using established chemistry is presented. Fmoc/tert-butyl-based protecting groups were used for the peptidic moieties in conjunction with acetyl sugar protection. Koenigs-Knorr glycosylations were carried out using protected bromomannose derivatives, the acceptor being threonine or serine, either in solution or within a resin-bound peptide. The characterisation of microgram quantities of glycopeptides by the use of glycosidases in combination with mass spectrometry is also described.Keywords
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