Uptake of 2-Oxoglutarate in Synechococcus Strains Transformed with the Escherichia coli kgtP Gene
- 1 January 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (1) , 211-215
- https://doi.org/10.1128/jb.182.1.211-215.2000
Abstract
A number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U- 14 C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U- 14 C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene.Keywords
This publication has 30 references indexed in Scilit:
- Obligate phototrophy in cyanobacteria: more than a lack of sugar transportFEMS Microbiology Letters, 1998
- Microbial genome analyses: global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities 1 1Edited by G. Von HeijneJournal of Molecular Biology, 1998
- Dephosphorylation of the phosphoprotein PII in Synechococcus PCC 7942: identification of an ATP and 2‐oxoglutarate‐regulated phosphatase activityMolecular Microbiology, 1997
- Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding RegionsDNA Research, 1996
- Nitrate and nitrite transport in the cyanobacterium Synechococcus sp. PCC 7942 are mediated by the same permeaseBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1994
- Molecular and genetical analysis of the fructose‐glucose transport system in the cyanobacterium Synechocystis PCC6803Molecular Microbiology, 1989
- A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkersGene, 1988
- Evidence for bicarbonate accumulation by Anacystis nidulansCanadian Journal of Botany, 1984
- Generic Assignments, Strain Histories and Properties of Pure Cultures of CyanobacteriaMicrobiology, 1979
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978