Incorporation of radioactive phosphate into lipids and pentosenucleic acid of cat brain slices. The effect of inorganic ions

Publisher Website
Google Scholar
Abstract
An increase in the concentration of K+, omission of Ca2+, or the addition of NH4+ to the incubating medium caused a decrease in the incorporation of [P32] phosphate into the lipid P, pentosenucleic acid (PNA), residue organic P (ROP) and phosphoprotein P(PP) of slices of cat brain respiring in a Krebs-Ringer bicarbonate buffer containing glucose and inorganic phosphate labeled with P32. The addition of L-glutamate or L-glutamine to the medium, either in the presence or the absence of glucose, caused a decrease in the incorporation of P32 into the lipid P, ROP and PP. The inhibition of P32 incorporation was not the result of changes in the specific activity of inorganic P, either in the medium or in the slice. The similarity between the conditions that adversely affect the incorporation of P32 into the P-containing fractions of brain slices and those that cause a failure of brain slices to maintain their concentration of phosphocreatine is commented upon.