1‐Hydroxy monocyclic carotenoid 3,4‐dehydrogenase from a marine bacterium that produces myxol
- 2 July 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 570 (1-3) , 184-188
- https://doi.org/10.1016/j.febslet.2004.05.085
Abstract
A crtD (1-HO carotenoid 3,4-dehydrogenase gene) homolog from marine bacterium strain P99-3 included in the gene cluster for the biosynthesis of myxol (3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene-3,1′,2′-triol) was functionally identified. The P99-3 CrtD was phylogenetically distant from the other CrtDs. A catalytic feature was its high activity for the monocyclic carotenoid conversion: 1′-HO-torulene (3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-1′-ol) was prominently formed from 1′-HO-γ-carotene (1′,2′-dihydro-β,ψ-caroten-1′-ol) in Escherichia coli with P99-3 CrtD, indicating that this enzyme has been highly adapted to myxol biosynthesis. This unique type of crtD is a valuable tool for obtaining 1′-HO-3′,4′-didehydro monocyclic carotenoids in a heterologous carotenoid production systemKeywords
This publication has 31 references indexed in Scilit:
- Genes Involved in the Biosynthesis of Photosynthetic Pigments in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicinaApplied and Environmental Microbiology, 2003
- Structural and functional analysis of a lycopene β‐monocyclase gene isolated from a unique marine bacterium that produces myxolFEBS Letters, 2003
- Myxoxanthophyll in Synechocystis sp. PCC 6803 is Myxol 2′-Dimethyl-Fucoside, (3R,2′S)-Myxol 2′-(2,4-di-O-Methyl-α-l-Fucoside), not RhamnosidePlant and Cell Physiology, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Growth and Feeding Habits in Reared Japanese Centropomid Fish Lates japonicus.NIPPON SUISAN GAKKAISHI, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of β‐caroteneFEBS Letters, 1993
- Isolation of a carotenoid biosynthesis gene coding for ζ-carotene desaturase fromAnabaenaPCC 7120 by heterologous complementationFEMS Microbiology Letters, 1993
- Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprintJournal of Molecular Biology, 1986
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982