Enterobacter aerogenes OmpX, a cation‐selective channel mar‐ and osmo‐regulated
- 7 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 569 (1-3) , 27-30
- https://doi.org/10.1016/j.febslet.2004.05.047
Abstract
The ompX gene of Enterobacter aerogenes was cloned. Its overexpression induced a decrease in the major porin Omp36 production and consequently a β-lactam resistance was noted. Purified outer membrane protein X (OmpX) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 pS in 1 M NaCl and a cationic selectivity. Both MarA expression and high osmolarity induced a noticeable increase of the OmpX synthesis in the E. aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non-specific porins, Omp36 and Omp35, decreasedKeywords
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