Mitogenic Properties of Rabbit Anti‐Human β2‐Microglobulin for Murine B Cells

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Abstract
Rabbit antisera against human β2‐microgluhulin were found to be mitogenic for mouse spleen cells, giving rise to a peak DNA synthetic response on day 2 in cultures containing serum‐free medium. The mitogenic effect was shown on cells from spleens of nude ‘athymic’ mice and on spleen B cells, but no effect was found on cortisone resistant mouse thymocytes or on spleen T cells. Thus, it was concluded that the serum was mitogenic for mouse B cells but not for T cells. This conclusion was confirmed by experiments showing that the antiserum was able to induce polyclonal antibody synthesis in mouse spleen cells in culture. The activity of the scrum was absorbed by pure human β2‐microglobulin as well as by mouse thymocytes and bone marrow cells