The enrichment of n−3 polyunsaturated fatty acids using aminopropyl solid phase extraction columns
- 1 January 1993
- Vol. 28 (1) , 51-54
- https://doi.org/10.1007/bf02536360
Abstract
A rapid, simple and reliable method is described for the preparation of concentrates of methyl or ethyl esters of n−3 polyunsaturated fatty acids by solid phase extraction using aminopropyl bonded silica columns. After applying mixtures of fatty acid esters in hexane, saturated and monounsaturated fatty acid esters are preferentially eluted with hexane whereas polyunsaturated fatty acids (PUFA) can subsequently be eluted with dichloromethane. Concentrates containing 80–90% n−3 PUFA can thus be obtained using fish oil fatty acids esters as a starting material.Keywords
This publication has 13 references indexed in Scilit:
- Silica Gel Thin-Layer Chromatographic Method for Concentration of Longer-Chain Polyunsaturated Fatty Acids From Food and Marine LipidsCanadian Institute of Food Science and Technology Journal, 1991
- Lipid and fatty acid composition is altered in plaque tissue from multiple sclerosis brain compared with normal brain white matterLipids, 1991
- Enrichment of eicosapentaenoic acid and docosahexaenoic acid esters from esterified fish oil by programmed extraction-elution with supercritical carbon dioxideJournal of Chromatography A, 1990
- Separation and Isolation ProceduresPublished by Springer Nature ,1986
- Effects of dietary polyunsaturated fatty acid deficiencies on mortality, growth and gill structure in the turbot, Scophthalmus maximusJournal of Fish Biology, 1985
- Rapid separation of lipid classes in high yield and purity using bonded phase columns.Journal of Lipid Research, 1985
- Fatty acid fractionation by column distillation: Purity, energy consumption and operating conditionsJournal of Oil & Fat Industries, 1984
- Separation and quantitation of free fatty acids and fatty acid methyl esters by reverse phase high pressure liquid chromatographyJournal of Lipid Research, 1983
- Fractionation of fish oil fatty acid methyl esters by means of argentation and reversed-phase high-performance liquid chromatography, and its utility in total fatty acid analysisJournal of Chromatography A, 1980
- Considerations of small particles in different modes of liquid chromatographyJournal of Chromatography A, 1974