IMPROVED POLYSTYRENE POLYMERIC XAD-2 RESIN COLUMN EXTRACTION OF 5-BETA-CHOLESTAN-3 BETA-OL FROM POLLUTED WATER

  • 1 January 1978
    • journal article
    • research article
    • Vol. 15  (2) , 67-73
Abstract
Appropriate methods and criteria for judging the degree of sewage contamination of water and its suitability for drinking or recreational uses are essential to safeguard the public health. 5 .beta.-Cholestan-3.beta.-ol seems to satisfy many, if not all, of the criteria required of a good indicator of fecal pollution. This fecal sterol was strongly adsorbed to the polystyrene polymeric XAD-2 adsorbents at low pH, resulting in a 100% retention. The adsorbed sterols could be easily removed from the columns with acetone adjusted to pH 8.5-9 with concentrated NH4OH. Large volumes of fresh and sea water samples can be extracted by the closed column method in a relatively short time. The sensitivity of the column exceeded that of the conventional liquid-liquid partitioning procedure. With some modifications, the column extraction process can be incorporated in a fully- or semi-automated analytical procedure.

This publication has 2 references indexed in Scilit: