Isolation of Three Novel Human Genes Encoding G Protein-Coupled Receptors
- 1 January 1995
- journal article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 14 (1) , 25-35
- https://doi.org/10.1089/dna.1995.14.25
Abstract
We have cloned and mapped the chromosomal location of three novel human genes encoding G protein-coupled receptors that we have named GPR6, GPR5, and GPR4. The entire coding region for each of these genes was contained on single exons. Gene GPR6 encoded a receptor that shared closest identity (71% in the transmembrane regions) with the human orphan receptor GPR3 and was localized to chromosome 6 (q21-q22.1). Northern blot analysis revealed that GPR6 transcripts were abundant in the human putamen and to a lesser extent in the frontal cortex, hippocampus, and hypothalamus. Gene GPR5 encoded a receptor that most closely resembled the orphan receptor RBS11 (48% in the transmembrane regions) and the MIP Iα/RANTES receptor (45% in the transmembrane regions) and was localized to chromosome 3 (p21.3-p21.1). Gene GPR4 shared identity (40% in the transmembrane regions) with the human platelet-activating factor receptor and was localized to chromosome 19 (q13.2-q13.3).link_to_subscribed_fulltexKeywords
This publication has 31 references indexed in Scilit:
- ORL1, a novel member of the opioid receptor familyPublished by Wiley ,2001
- Desensitization, phosphorylation and palmitoylation of the human dopamine D1 receptorEuropean Journal of Pharmacology: Molecular Pharmacology, 1994
- Molecular cloning of a novel putative G protein‐coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous systemFEBS Letters, 1993
- Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4Biochemical and Biophysical Research Communications, 1993
- Molecular cloning of a novel rat G-protein-coupled receptor gene expressed prominently in lung, adrenal, and liverFEBS Letters, 1993
- Cloning, expression, and characterization of a gene encoding the human angiotensin II type 1a receptorBiochemical and Biophysical Research Communications, 1992
- Human dopamine D5 receptor pseudogenesGene, 1991
- Cloning, sequencing and tissue distribution of a candidate G protein‐coupled receptor from rat pituitary glandFEBS Letters, 1991
- Characterization of a human cDNA that encodes a functional receptor for platelet activating factorBiochemical and Biophysical Research Communications, 1991
- Molecular cloning of the human histamine H2 receptorBiochemical and Biophysical Research Communications, 1991