The Effects of X-Rays on the Progress of L-Cells through the Cell Cycle

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Abstract
The effects of x-rays on the progress of the L60TM subline of Earle''s L-cells through the stages of the deoxyribonucleic acid (DNA) synthesis cycle were studied in cell culture using isotopically labeled specific DNA precursors and autoradiography. The progress of cells through all portions of the cycle was affected by x-rays. The effects observed included the following depression in the rate of progress of cells from the pre-DNA synthesis (G1) phase to the DNA synthesis (S) phase; depression of the rate of DNA synthesis shortly after irradiation; recovery of rate of DNA synthesis at later times; inhibition of progress of cells from the S phase into the post-DNA synthesis (G2) phase; reversal of this inhibition; arrest of the progress of cells out of the G2 phase. Thus, many of the radiation effects on the mammalian cell cycle described by various workers using different cell systems have been demonstrated in a single cell culture strain.