The Determination of Polycyclic Aromatic Hydrocarbons in Indigenous and Transplanted Mussels (Mytilys EdulisL.) Along the Dutch Coast

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Abstract
A method, originally developed to investigate the pollution of Dutch coastal water with metals and PCBs,1 was modified for the determination of the pollution with polycyclic aromatic hydrocarbons (PAH). The method is based on active biological monitoring with mussels (Mytilus edulis L.). Its usefulness has already been demonstrated.2 In the present study a method for the determination of PAH in mussels has been developed. The method is based on the hydrolysis of tissue with 4 M sodium hydroxide, extraction with hexane, clean-up with 10% deactivated aluminium oxide and quantitative determination with Reversed Phase High Performance Liquid Chromatography (RP-HPLC) and fluorescence detection. The accumulation plateau of most of the PAH studied has not been reached after 60 days. Gradients of pollution were found, and at least one significant source near IJmuiden was detected.

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