INFLUENCE OF TEMPERATURE ON DETERMINATION OF ENZYME-ACTIVITIES IN HUMAN-SERUM GAMMA-GLUTAMYL TRANSFERASE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 22  (4) , 213-218
Abstract
Optimum reaction conditions for determination of .gamma.-glutamyl transferase [EC 2.3.2.2] were studied at 25, 30 and 37.degree. C using a kinetic test and .gamma.-glutamyl-3-carboxy-4-nitranilide as substrate. There was no dependence on temperature for half saturation constants of .gamma.-glutamyl-3-carboxy-4-nitranilide. The corresponding constants for glycylglycine were influenced by temperature and pH. The pH optimum showed a dependence on temperature. In Arrhenius'' plot, straight line deviation can be observed only above 35.degree. C. The influence of temperature on the determination of enzyme activities in human serum are discussed.

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