LOCALIZATION OF THE G-CSF GENE ON CHROMOSOME 17 PROXIMAL TO THE BREAKPOINT IN THE T(15,17) IN ACUTE PROMYELOCYTIC LEUKEMIA

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  • 1 July 1987
    • journal article
    • research article
    • Vol. 70  (1) , 330-332
Abstract
The human granulocyte-colony stimulating factor gene (G-CSF) is localized at 17q11.2-q21, the region of one of the breakpoints in the 15;17 chromosome translocation specific for acute promyelocytic leukemia (APL). As G-CSF induces differentiation and loss of tumorigenicity in myeloid leukemic cells or cell lines, it was possible that the translocation in APL involved the DNA of the G-CSF coding region or its regulatory region. In situ hybridization to chromosomes with the t(15;17) from patients with the APL translocation using a G-CSF cDNA clone revealed that the coding region of this gene is proximal to the t(15;17) breakpoint on chromosome 17. Southern analysis of DNA from patients with the APL translocation showed no differences in hybridization between normal and leukemic cells. These results indicate that the G-CSF coding sequences is not disrupted by the chromosomal rearrangement characteristic of APL.

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