Selectivity of Melatonin Pituitary Inhibition for Luteinizing Hormone-Releasing Hormone

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Abstract
The pineal indole melatonin suppresses the neonatal rat luteinizing hormone (LH) and follicle-stimulating hormone (FSH) responses to LH-releasing hormone (LHRH), as shown in previous studies from this laboratory. We show in this study that the melatonin inhibition is a selective effect and is not due to general inhibition of pituitary function. The effects of the indole on the responses to thyrotropin-releasing hormone (TRH) and somatostatin (SRIF) and on basal pituitary hormone secretion were examined with cells in culture. Neonatal rat anterior pituitary cells dissociated with collage-nase and hyaluronidase were cultured overnight and distributed to 35-mm dishes at the time of use. For examination of melatonin effects on the response to releasing hormones, the cells were incubated for 3 h in control medium or medium containing LHRH (10–9-10–6M), TRH (10–10-10–6M), or SRIF (10–9-10–6M), either alone or in the presence of melatonin (10–8 or 10–6M). For examination of basal hormone secretion, the cells were incubated for 1.5, 3, 6, 15, or 24 h in either medium alone or medium containing melatonin (10–6M). Medium and cell lysate concentrations of LH, FSH, thyroid-stimulating hormone (TSH), prolactin (PRL) and growth hormone (GH) were determined by double antibody RIA. As previously, melatonin (10–8M) significantly suppressed LH and FSH release by all concentrations of LHRH. This concentration of the indole produced maximal suppression of both LH and FSH responses to LHRH.By contrast, melatonin at a 100-fold greater concentration (10–6M) had no effect on TRH stimulation of TSH or PRL release or on SRIF inhibition of GH release. Similarly, melatonin had no effect on basal release of TSH, PRL, or GH at the times examined. These findings show that melatonin inhibition of the gonadotroph response to LHRH is a selective effect.