Substrate structural requirements of Schizosaccharomyces pombe RNase P
- 17 July 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2) , 84-88
- https://doi.org/10.1016/0014-5793(89)81433-4
Abstract
RNase P from Schizosaccharomyces pombe has been purified over 2000‐fold. The apparent K m for two S. pombe tRNA precursors derived from the supS1 and sup3‐e tRNASer genes is 20 nM; the apparent V max is 2.5 nM/min (supS1) and 1.1 nM/min (sup3‐e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.Keywords
This publication has 11 references indexed in Scilit:
- Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P fromSaccharomyces cerevisiaeNucleic Acids Research, 1988
- Yeast RNase P: catalytic activity and substrate binding are separate functions.Proceedings of the National Academy of Sciences, 1988
- Role of the Protein Moiety of Ribonuclease P, a Ribonucleoprotein EnzymeScience, 1988
- Cleavage of RNA by RNase P from Escherichia coliPublished by Elsevier ,1987
- Alteration of a mitochondrial tRNA precursor 5′ leader abolishes its cleavage by yeast mitochondrial RNase PNucleic Acids Research, 1987
- Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA sequences.Proceedings of the National Academy of Sciences, 1986
- A single base change in the intron of a serine tRNA affects the rate of RNase P cleavage in vitro and suppressor activity in vivo in Saccharomyces cerevisiae.Journal of Biological Chemistry, 1986
- Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.Molecular and Cellular Biology, 1985
- The RNA moiety of ribonuclease P is the catalytic subunit of the enzymePublished by Elsevier ,1983