Effect of PAN on Certain Photosynthetic Reactions

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Abstract
The effect of the atmospheric oxidant, peroxyacetyl nitrate (PAN) on photosynthetic phosphorylation, DADP+ reduction, and CO2 fixation by chloroplasts prepared from spinach and bean plants was studied. When this gaseous oxidant was passed through reaction mixtures in the light, inhibition of the photosynthetic reactions occurred. Inhibition in ATP and NADPH formation and the fixation of C O14 2 was prevented by the addition of ascorbate to the reaction mixture. Also NADP+ reduction was maintained by adding either excess photosynthetic pyridine nucleotide reductase (PPNR) or heat inactivated PPNR preparations. Gassing the individual components, chloroplasts, or PPNR in the light or dark, prior to their use in a complete reaction, did not cause inhibition.