The COP9 Signalosome Interacts with SCFUFO and Participates in Arabidopsis Flower Development
Open Access
- 10 April 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 15 (5) , 1071-1082
- https://doi.org/10.1105/tpc.009936
Abstract
The COP9 signalosome (CSN) is involved in multiple developmental processes. It interacts with SCF ubiquitin ligases and deconjugates Nedd8/Rub1 from cullins (deneddylation). CSN is highly expressed in Arabidopsis floral tissues. To investigate the role of CSN in flower development, we examined the expression pattern of CSN in developing flowers. We report here that two csn1 partially deficient Arabidopsis strains exhibit aberrant development of floral organs, decline of APETALA3 (AP3) expression, and low fertility in addition to defects in shoot and inflorescence meristems. We show that UNUSUAL FLORAL ORGANS (UFO) forms a SCFUFO complex, which is associated with CSN in vivo. Genetic interaction analysis indicates that CSN is necessary for the gain-of-function activity of the F-box protein UFO in AP3 activation and in floral organ transformation. Compared with the previously reported csn5 antisense and csn1 null mutants, partial deficiency of CSN1 causes a reduction in the level of CUL1 in the mutant flowers without an obvious defect in CUL1 deneddylation. We conclude that CSN is an essential regulator of Arabidopsis flower development and suggest that CSN regulates Arabidopsis flower development in part by modulating SCFUFO-mediated AP3 activation.Keywords
This publication has 55 references indexed in Scilit:
- Multiple Ubiquitin Ligase–Mediated Processes Require COP9 Signalosome and AXR1 FunctionPlant Cell, 2002
- The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysisJournal of Cell Science, 2002
- Deletion Mutants in COP9/Signalosome Subunits in Fission YeastSchizosaccharomyces pombeDisplay Distinct PhenotypesMolecular Biology of the Cell, 2002
- The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p.2001
- Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCF TIR1 in Mediating Auxin ResponseScience, 2001
- Activation of the Arabidopsis B Class Homeotic Genes byAPETALA1Plant Cell, 2001
- The subunit 1 of the COP9 signalosome suppresses gene expression through its N-terminal domain and incorporates into the complex through the PCI domainJournal of Molecular Biology, 2001
- The Arabidopsis floral homeotic gene APETALA3 differentially regulates intercellular signaling required for petal and stamen developmentDevelopment, 2001
- SCF and Cullin/RING H2-Based Ubiquitin LigasesAnnual Review of Cell and Developmental Biology, 1999
- Making sense of the COP9 signalosome: a regulatory protein complex conserved from Arabidopsis to humanTrends in Genetics, 1999