A study of the bile pigments in relation to the van den bergh reaction

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Abstract
Bilirubin when suspended in normal human or ox-serum does not give a direct diazo-re-action, but gives the indirect reaction. Fresh bile contains a pigment which yields a direct reaction if it is previously dissolved in serum. The authors suggest the term "direct-reaction pigment" for this substance. Directions are described for isolating the substance in an impure state. The properties of the substance are different from those of bilirubin. In the sera which give an indirect reaction the pigment apparently is bilirubin. The "direct reaction pigment" is present in those sera which give a direct van den Bergh reaction.

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