Monitoring fibroblast behavior in tissue culture with an applied electric field.

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Abstract
Mammalian fibroblasts have been cultured on evaporated gold electrodes subjected to an alternating electric field at 4000 Hz. The system consists of a large (approximately equal to 2 cm2) and a small (approximately equal to 3 X 10(-4) cm2) electrode bathed in tissue culture medium. The applied electric field produces a voltage drop at the boundary between the solution and the small electrode of a few mV at a current density of a few mA/cm2. The small population of cells that attach and spread on this electrode have a marked effect on the measured impedance and also cause it to fluctuate with time. The amplitude of these fluctuations is greatly reduced by cytochalasin B (10 microM), suggesting they are a consequence of cell movement.

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