HUMAN NEUTROPHIL AGGREGATION AND INCREASED ADHERENCE TO HUMAN-ENDOTHELIAL CELLS INDUCED BY HEAT-AGGREGATED IGG AND IMMUNE-COMPLEXES

  • 1 January 1981
    • journal article
    • research article
    • Vol. 44  (3) , 538-547
Abstract
Several types of IgG-dependent phagocytic stimuli independent of complement were investigated for their property to induce human polymorphonuclear neutrophil leukocyte (PMN) aggregation and adherence to human endothelial cells (EC) in culture. A Coulter counter method was employed for cell aggregation detection. Aggregated IgG, ovalbumin anti-ovalbumin (OV anti-OV) immune complexes (both insoluble and soluble) and opsonized latex particles induced a significant PMN aggregation degree which was detectable as early as 2 min after exposure of PMN to these stimuli. This aggregation was dependent on divalent cations (Ca2+, Mg2+). The same phagocytic stimuli furthermore significantly increased PMN adherence to cultured human EC and serum-coated plastic. Controls consisting of native IgG, and OV and anti-OV complexes prepared from F(ab'')2 antibody failed to induce either aggregation or increased adherence of PMN. PMN exposure to IgG-dependent phagocytic stimuli induces increased PMN adhesiveness and interaction between the Fc-receptor of PMN and the Fc-portion of phagocytic stimuli is essential for this effect.