Biochemical Characterization of the Mitochondrial Permeability Transition in Isolated Forebrain Mitochondria
- 1 December 2000
- journal article
- Published by S. Karger AG in Developmental Neuroscience
- Vol. 22 (5-6) , 376-383
- https://doi.org/10.1159/000017463
Abstract
Induction of the mitochondrial permeability transition (PT) has been proposed to contribute to neuronal cell death. Nearly all studies of the biochemistry of PT induction, however, have been conducted in isolated liver mitochondria. To better understand PT induction in brain mitochondria, we used Ficoll gradients to purify nonsynaptosomal mitochondria from the forebrains of male Fischer 344 rats. Incubation of these mitochondria with Ca2+ was associated with a loss of absorbance. Inorganic phosphate enhanced this loss of absorbance, and the PT inhibitor cyclosporin A reduced it, especially in conjunction with ADP. These findings suggest that Ca2+-mediated loss of absorbance resulted from PT induction. Na+, which enhances mitochondrial Ca2+ efflux, but stimulates mitochondrial free radical production, had no effect on PT induction. These data confirm the existence of tissue-specific differences in the nature of PT induction.Keywords
This publication has 19 references indexed in Scilit:
- Dopamine Oxidation Alters Mitochondrial Respiration and Induces Permeability Transition in Brain MitochondriaJournal of Neurochemistry, 1999
- Mitochondria and ApoptosisScience, 1998
- Apoptosis Induction by Caspase-8 Is Amplified through the Mitochondrial Release of Cytochrome cJournal of Biological Chemistry, 1998
- Regulation of the Permeability Transition Pore in Skeletal Muscle MitochondriaJournal of Biological Chemistry, 1998
- CorrespondenceNeuroscience, 1996
- The Cytotoxicity of Tumor Necrosis Factor Depends on Induction of the Mitochondrial Permeability TransitionJournal of Biological Chemistry, 1996
- Modulation of the Mitochondrial Permeability Transition Pore by Pyridine Nucleotides and Dithiol Oxidation at Two Separate SitesJournal of Biological Chemistry, 1996
- 4-Hydroxyhexenal Is a Potent Inducer of the Mitochondrial Permeability TransitionJournal of Biological Chemistry, 1996
- Interactions of Cyclophilin with the Mitochondrial Inner Membrane and Regulation of the Permeability Transition Pore, a Cyclosporin A-sensitive ChannelJournal of Biological Chemistry, 1996
- Permeabilization of the inner mitochondrial membrane by Ca2+ ions is stimulated by t-butyl hydroperoxide and mediated by reactive oxygen species generated by mitochondriaFree Radical Biology & Medicine, 1995