Voltammetric Determination of Lead Labelled Albumin and of Albumin Antiserum by Immunoassay

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Abstract
Differential pulse polarography was utilized in voltammetric immunoassay to determine human albumin and albumin antiserum. Albumin was labeled with Pb, which exhibited a reduction peak with E1/2 [half-wave potential] at -680 mV vs. SCE [saturated calomel electrode]. The current is proportional to the concentration of Pb labeled albumin, and hence the albumin at a fixed Pb concentration, and it decreases in proportion to the amount of albumin antiserum added. Albumin concentrations of 2.0 .times. 10-6 to 10 .times. 10-6 M and 10-50 .mu.l of goat albumin antiserum (titer 1:16 = maximum dilution causing precipitation with equal volume of 1 mg/ml human albumin) were measured in the presence of 2 .times. 10-6 M Pb.

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