Sterility Test Method for Petrolatum-Based Ophthalmic Ointments
Open Access
- 1 January 1970
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 20 (5) , 798-801
- https://doi.org/10.1128/aem.20.5.798-801.1970
Abstract
A sensitive sterility testing procedure for the detection of microbial contamination in petrolatum-based ointments is described. The method involves dissolving the ointment in filter-sterilized isopropyl myristate and filtering through a membrane filter. Improved sensitivity is obtained by blending the membrane in Trypticase Soy Broth before incubation. Filter-sterilized isopropyl myristate is shown to be less toxic to microorganisms than heat-sterilized isopropyl myristate. The isopropyl myristate method is more sensitive than the polyethylene glycol-ether method for the detection of microbial contamination.Keywords
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