Inhibition of 3H-Thymidine Incorporation into Human Lymphocytes by Dialyzable Material of Streptokinase-Streptodornase
- 1 January 1976
- journal article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 50 (4) , 402-415
- https://doi.org/10.1159/000231530
Abstract
Using the 3H-thymidine incorporation test, the lymphocyte-stimulating activity of streptokinase-streptodornase (SK-SD, Varidase) and its fractions were evaluated. The dose-response curves of all the preparations except the third fraction had two peaks with the maximum around 5 and 40 μg. The dialyzable fraction of Sk-SD (D-SK-SD) occupying approximately half of the original sample had almost no lymphocyte-stimulating activity; and conversely, it had a potent inhibitory effect on the 3H-thymidine incorporation into lymphocytes that normally occurs with non-dialyzable SK-SD (ND-SK-SD), PPD and PHA-P. Therefore, it will be desirable to remove the dialyzable fraction from the commercial SK-SD preparation when it is used for the determination of the capacity of cellular immunity against SK-SD.Keywords
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