Absence of low molecular weight DNA polymerase activity from the nuclei ofAmoeba discoides
- 1 April 1978
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 34 (4) , 427-428
- https://doi.org/10.1007/bf01935903
Abstract
Summary Amoeba discoides nuclear protein partially purified by passage through Sephadex G-200 showed 3 high-mol.-wt DNA polymerase activities which eluted in and just following the void volume. No low-mol.-wt (45,000 daltons) DNA polymerase β activity was detected. Nuclear protein layered on 5–20% sucrose gradients also showed an absence of lowmol.-wt DNA polymerase β. The void volume enzyme showed deoxyribonuclease activity, but no low-mol.-wt nuclease activity was detected.Keywords
This publication has 15 references indexed in Scilit:
- DNA polymerase of Dictyostelium discoideumBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Phylogeny of DNA Polymerase-βScience, 1976
- Vertebrate DNA polymerasesCell, 1975
- Deoxyribonucleic acid polymerases of BHK-21/C13 cells. Partial purification and characterization of the enzymesBiochemical Journal, 1975
- DNA-dependent DNA polymerase activities from Paramecia macronucleiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- DNA polymerases of Euglena gracilis: heterogeneity of molecular weight and subunit structureNucleic Acids Research, 1975
- Deoxyribonucleic Acid Polymerases from YeastEuropean Journal of Biochemistry, 1974
- The interrelation between DNA synthesis and various DNA polymerase activities in synchronized HeLa cellsJournal of Molecular Biology, 1974
- [7] Sea urchin nuclear DNA polymeraselPublished by Elsevier ,1974
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961