Effect of Synthetic Human Atrial Natriuretic Peptide on Aldosterone Secretion by Dispersed Aldosterone-Producing Adenoma Cellsin Vitro*

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Abstract
The effect of synthetic α-human atrial natri-uretic peptide (αhANP), a potent natriuretic and vasorelaxant polypeptide recently isolated from human atria, on aldosterone secretion was studied in vitro in collagenase-dispersed adrenal adenoma cells from a patient with primary aldosteronism. ahANP (3.2 × 10–7 M) signficantly inhibited both basal and potassium (16 mM)-stimulated aldosterone secretion, whereas it had little or no effect on aldosterone secretion submaximally or maximally stimulated by ACTH (3.4 × 10–10–3.4 × 10–9 M) or angiotensin II (10–8–10–9 M). The less potent effect of ahANP on aldosterone secretion by dispersed human adrenal tumor cells compared to that in in vitro animal studies may reflect decreased affinity and/or number of specific receptors for ANP on the tumor cells. Whether ANP plays a physiological role in regulation of aldosterone secretion in humans in vivo remains to be determined.