Phospholipase D activity of Corynebacterium pseudotuberculosis (Corynebacterium ovis) and Corynebacterium ulcerans, a distinctive marker within the genus Corynebacterium

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Abstract
A search was made for corynebacterial phospholipase D, ovis toxin, a sphingomyelinase (phosphatidylcholine phosphohydrolase), among a wide variety of corynebacteria. Phospholipase D activity was found in strains exhibiting the biochemical properties characteristic of C. pseudotuberculosis or of C. ulcerans and in no other species of Corynebacterium. Methods for the assay of phospholipase D as a sphingomyelinase and methods for screening for phospholipase D in the presence of C. equi on washed sheep blood agar are discussed.