Modulation of functional activities of the neurotoxin from black widow spider venom
- 27 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (2) , 205-207
- https://doi.org/10.1016/0014-5793(93)80803-3
Abstract
We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [ 14 C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [ 14 C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.Keywords
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