A Simple, Rapid, High Yield Isolation and Purification Procedure for Chloroperoxidase Isoenzymes
- 5 December 1985
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 15 (5) , 335-348
- https://doi.org/10.1080/00327488508062450
Abstract
A simple four-step procedure has been developed for Isolation of chloroperoxldase from the mold Caldarlomyces fumago. Polyethyleneglycol precipitation of the contaminating pigment in the growth medium, followed by chromatography of the soluble enzyme fraction on a QAE-ZetaPrep-250 cartridge and ammonium sulfate precipitation affords Isolation of the chloroperoxldase. Extensive dialysis and chromatography on DE-53 cellulose allows the separation and further purification of chloroperoxldase A and B isoenzymes.Keywords
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