THE BINDING OF ACETYLCHOLINE TO SYNAPTIC VESICLES FROM TORPEDO CALIFORNICA ELECTROPLAX
- 4 October 1976
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 27 (4) , 867-872
- https://doi.org/10.1111/j.1471-4159.1976.tb05148.x
Abstract
Abstract— The in vitro uptake of exogenous acetylcholine by isolated presynaptic vesicles has been demonstrated in a new system. A preparation of vesicles from Torpedo californica electroplax was developed in which acetylcholinesterase and acetylcholine receptor activity were blocked. The vesicles bound acetylcholine with Kd 1.58 μM, the maximum amount bound being 26 pmol per g of original tissue, or 52 molecules per vesicle. Nicotinic drugs blocked binding, but muscarinic and noncholinergic drugs did not. The relative potency of nicotinic drugs differed greatly from their potency on Torpedo receptor. Sephadex chromatography showed that 26% of the binding was irreversible. The relationship of the binding to acetylcholine uptake and storage was discussed.This publication has 25 references indexed in Scilit:
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