Utilization of Tetrathionate and 35S-labelled Thiosulphate by Washed Cells of Chlorobium vibrioforme f. sp. thiosulfatophilum

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Abstract
Summary: The utilization of S4O2- 6 by washed cells of Chlorobium vibrioforme f. sp. thiosulfatophilum was light-dependent. The stoichiometry for S4O2- 6 oxidation and 14CO2 assimilation was 1:3·5. Both processes were inhibited by O2, but this effect was partially reversed by flushing the reaction mixture with argon. A variety of inhibitors of thiol groups and electron transport, as well as uncouplers and ionophores, restricted these activities. During the oxidation of 35S-labelled S2O2- 3 by washed cells, the sulphone S atom (from S35SO2- 3) was rapidly oxidized to SO2- 4 via SO2- 3, whereas the first identifiable product of the oxidation of the sulphane S atom (from 35SSO2- 3) was S2- and then elemental sulphur, which was extruded into the medium and then reutilized and oxidized to SO2- 4. A number of inhibitors of thiol groups and respiratory carriers, as well as ionophores, also restricted the oxidation of S2O2- 3.

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