Cytochemistry of Marrow and Extramedullary Adipocytes in Monolayer Cultures

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Abstract
The behavior of isolated, disaggregated white adipose cells obtained from marrow and extramedullary sites in the rabbit was compared in monolayer cultures. Both cell types transform within 2 wk into fibroblast-like cells which can be maintained in subcultures. Both in freshly isolated state and in the fibroblast-like state the marrow adipocyte contains esterase activities reacting with naphthol AS acetate and D-chloracetate. These activities are not present in extramedullary cells. .alpha.-Naphthyl butyrate-reacting esterase, present in both cell types, is fluoride-resistant in marrow adipocytes but labile in extramedullary cells. These differences are consistent with heterogeneity of white adipose tissue and suggest different potentials for marrow and extramedullary adipocytes.