Morphological Changes in Rat Glioma Cells caused by Adenosine Cyclic 3', 5'-Monophosphate

1 January 1978

journal article
research article
Published by Japan Society for Cell Biology in Cell Structure and Function

Vol. 3 (2) , 103-112
https://doi.org/10.1247/csf.3.103

Abstract

Morphological changes in a cell line from rat glioma induced in vitro by exposure to dibutyryl adenosine cyclic 3', 5'-monophosphate (db-cAMP) and other agents were studied. Db-cAMP, adenosine cyclic 3', 5'-monophosphate (cAMP) plus theophylline or cholera toxin plus theophylline caused shrinkage of parts of the cytoplasm and converted apparently immature cells to cells with oligodendroglial or astroglial morphology. This morphological alteration induced by db-cAMP proceeded rapidly and was completed within an hour. The morphological alteration induced by db-cAMP and cAMP was reversible, but that mediated by cholera toxin was not reversible. Cytochalasin B also induced a morphological alteration similar to that induced by db-cAMP. Agents such as colcemid counteracted the effects of db-cAMP, cAMP and cholera toxin. These findings suggest that the intra-cellular level of cAMP may affect the organization of microtubules and resultin inducting morphological changes in this glial cell line.

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