Measurement of Human and Mouse Interleukin‐12
Open Access
- 1 September 1995
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 15 (1) , 6.16.1-6.16.15
- https://doi.org/10.1002/0471142735.im0616s15
Abstract
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts (“PHA blasts”). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.Keywords
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