Rate of glucose transformation in the rat by whole-body analysis after blucose-14-C

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Abstract
Rate of disposal of glucose in the normal fasted rat, as calculated by Stewart-Hamilton dilution principle after a single injection of glucose-U-14C, was 0.22 mg glucose carbon/min. per 100 g rat. Analysis of the homogenized whole animal showed that rate of conversion to CO2 was 0.13 mg/min. (61% of total disposal), glycogen 0.017, protein 0.029, lipid 0.011, and urine 0.029. The percentage of CO2 derived from glucose was 10%. Activity was recovered in a variety of subtractions of lipid. Urinary activity was chiefly in unidentified acidic compounds. Although multi-compartmental analysis was not attempted, the behavior of several pools was characterized. Tracer glucose equlibrated so promptly with total body glucose that a single pool would be appropriate in a kinetic model. Composite moieties of acidic and basic compounds received 14C very rapidly; however, neither class of compounds behaved as a single pool. Specific activity curves for serum, muscle, and brain suggested that extracellular and intracellular bicarbonate compartments for the body as a whole would not be appropriate in an over-all model.