Electrochemical Detection of Benperidol in Serum for Drug Monitoring in Humans
- 1 September 1987
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 9 (3) , 343-346
- https://doi.org/10.1097/00007691-198709000-00015
Abstract
A high-pressure liquid chromatographic (HPLC) method for the serum assay of benperidol is described. One ml of serum is required for a single estimation. The method involves a simple and rapid extraction step (BondElut columns), HPLC separation (C8 10-.mu. column), and electrochemical detection ( + 0.65 V). Haloperidol is used as internal standard. On the basis of this procedure, recovery (93-97%) and reproductivity (intra-assay and inter-assay coefficients of variation < 9%) are satisfactory. The detection limit is 0.2 ng/ml of serum. After therapeutic doses, trough serum levels ranged from 3.8 to 12 ng/ml in five patients.This publication has 0 references indexed in Scilit: