Gene repertoire of the anti-poly(Glu60Ala30Tyr10) (GAT) immune response: comparison of VH, V kappa, and D regions used by anti-GAT antibodies and monoclonal antibodies produced after anti-idiotypic immunization.

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Abstract
Eight monoclonal antibodies were selected from BALB/c mice immunized with 2 different monoclonal anti-idiotypic antibodies recognizing 2 discrete idiotopes characteristic of the anti-poly(Glu60 Ala30 Tyr10) (GAT) antibody response. These monoclonal antibodies were previously classified as Ab''1 (anti-GAT-like) and Ab3 (anti-anti-idiotype) on the basis of expression of the public idiotypic specificity (p.GAT) studied with a xenogeneic serum, anti-GAT activity, and expression of various public idiotypes. All the H chain variable region (VH) sequences from ab''1 are nearly identical to the VH sequences of Ab1 anti-GAT monoclonal antibodies. The same type of result has been found with the Ab''1 K.kappa. light chain variables region (V.kappa.) sequences. Confirming this classification, Ab3 VH and V.kappa. sequences were found to be completely different, from Ab1 VH and V.kappa. sequences. The Ab1'' diversity (D) regions are different from one another and different from the D regions found on monoclonal anti-GAT antibodies but function similarly. These D regions are not simply derived from already described D genes. These results suggest that in the Anti-GAT response VH and V.kappa. sequences are mainly responsible for idiotype expression.

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