Alkaline Phosphatase and Osteogenesis in vitro.

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Abstract
Summary A study has been made of the role of alkaline phosphatase in the osteogene-sis of 7-day embryonic chick femora during 21 days in organ culture. The femora were analyzed for their content of inorganic P, acid-soluble ester P, lipid P, total nucleic acid P and residual P, after increasing culture periods. The results indicate that there is a close parallel between inorganic P content and alkaline phosphatase activity. Acid soluble ester P content shows a roughly reciprocal relationship to phosphatase activity, while the other P fractions do not appear to be related.