Metabolitic Pathway of Bisphenol a by Pseudomonas paucimobilis Strain FJ-4.

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Abstract
Metabolites occurring during bisphenol A (BPA) degradation by a bacterial strain Pseudomonas paucimobilis FJ-4 were identified by using liquid chromatography (HPLC) and gas chromatography/mass spectrometry (GC-MS) in order to clarify the BPA metabolic pathway in this strain, resulting in the detection of p-hydroxyacetophenone (p-HAP), p-hydroxybenzaldehyde (p-HBAL), p-hydroxybenzoic acid, 1, 2-bis (4-hydroxyphenyl) -2-propanol, 2, 2-bis (4-hydroxyphenyl) -1-propanol, 2, 3-bis (4-hydroxyphenyl) -1, 2-propanediol, p-hydroxyphenacyl alcohol, 2, 2-bis (4-hydroxyphenyl propanoic acid, and 4, 4-dihydroxyl-α-methylstilbene. Among them, p-HAP and p-HBAL were main metabolites: 0.8 mole of each was formed when l mole of BPA was removed, after which both metabolites were completely removed. This suggests that BPA is mainly degraded by the cleavage of its propane part to produce p-HAP and p-HBAL, followed by the complete degradation of both metabolites (main pathway) . On the other hand, 2, 3-bis (4-hydroxyphenyl) -1, 2-propanediol and p-hydroxyphenacyl alcohol were accumulated in the culture during BPA degradation though small amounts. As they have structures which cannot be produced by the cleavage of the propane part of BPA, it is suggested that there is another pathway in which the propane part is oxidized without its cleavage (side pathway) . Interestingly, these side pathway metabolites could not removed by the prolonged cultivation, indicating that side pathway seems to produce more recalcitrant metabolites than the original chemical BPA.

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