cAMP radioimmunoassay without interference from calcium or EDTA.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 28 (1) , 150-152
- https://doi.org/10.1093/clinchem/28.1.150
Abstract
Calcium and EDTA interfere with radioimmunoassay of cAMP or acetylated cAMP. Both interferences with the assay of acetylated cAMP can be obviated by including in the buffer 15 mmol of CaCl2 per liter, which at the same time increases the affinity of the antibody by 89%. As a result, antibody can be decreased by 65%. This method requires no prepurification (extraction or chromatography), and no correction for analytical recovery is necessary. This assay can detect 12 fmol of cAMP per tube, so only microliter quantities of plasma are needed.This publication has 4 references indexed in Scilit:
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- An ultrasensitive method for the simultaneous determination of cyclic AMP and cyclic GMP in small-volume samples from blood and tissueBiochemical Medicine, 1977
- Nephrogenous Cyclic Adenosine Monophosphate as a Parathyroid Function TestJournal of Clinical Investigation, 1977
- Radioimmunoassay for adenosine 3', 5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in human blood, urine, and cerebrospinal fluid.Clinical Chemistry, 1977