A Method for Long-time Culture Storage ofRhizoctonia solani

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Abstract
A method is described for long-time preservation of cultures of R. solani in tubes in a dry soil containing 4% (wt/wt) wheat bran. The moisture level of the mixture at the time of inoculation with R. solani was 0.23 g water per gram (dry weight) of soil. About 1 mo. at 23-27.degree. C was required to allow the colonized mix to air dry; then it was stored at -25.degree. C. Isolates from diverse sources survived 55 mo. at -25.degree. C. Virulence [radish (Raphanus sativus L. cv. Cherry Belle) seed germination assay], morphology and cultural characteristics of R. solani were not changed.

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