Construction of Genes or Gene Fragments by Use of Two Long Synthetic Oligonucleotides Representing the Coding and Noncoding Strands
- 1 August 1988
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 7 (4) , 497-510
- https://doi.org/10.1080/07328318808075392
Abstract
The construction of genes as exemplified for a gene encoding salmon calcitonin-gly(33) from only two long synthetic oligonucleotides (coding/noncoding strand) is described. The type of solid support used in oligonucleotide preparation strongly influences synthesis yields and mutation rates in the cloning of the synthetic DNA.This publication has 24 references indexed in Scilit:
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