Isolation and characterization of turkey prolactin

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Abstract
Prolactin [PRL] from frozen turkey pituitary glands was purified by alkaline ethanol extraction of the pituitary residue after pre-extractions of the gonadotropins and growth hormone, followed by gel filtration on Sephadex G-100 and DEAE-cellulose column chromatography at different pH. A specific radioligand-receptor assay for ovine prolactin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified turkey PRL was characterized by a radioligand-receptor assay to be 3.40 IU/mg and by a heterologous radioimmunoassay to be 35 IU/mg as compared to purified ovine PRL as standard. The yield of turkey PRL was 3 mg/100 g frozen pituitary glands. The purified turkey PRL consisted of 3 protein bands upon polyacrylamide gel electrophoresis and the MW was 22,000 by sodium dodecyl sylfate-polyacrylamide gel electrophoresis. Turkey PRL was rich in aspartic acid, glutamic acid and leucine, but lacking in methionine.