Characterization of Solubilized Proteins from Tissue Culture-and Host-Derived Nuclear Polyhedra of Lymantria dispar and Autographa californica
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 11 (3) , 174-181
- https://doi.org/10.1159/000149030
Abstract
A proteolytic activity capable of degrading solubilized proteins has been detected by SDS-PAGE in alkaline carbonate-chloride dissolutions of polyhedra isolated from the larvae of Lymantria dispar and Autographa californica. This activity could not be detected in dissolutions of the respective tissue culture-derived polyhedra. However, tissue culture-derived polyhedra passaged once through their respective hosts displayed the proteolytic activity. Proteolytic degradation was prevented by either heating an aqueous suspension of polyhedra at 70° for 20 min prior to dissolution or adding a proteolytic enzyme inhibitor (phenylmethylsulfonyl fluoride) to the dissolutions.Keywords
This publication has 4 references indexed in Scilit:
- Electrophoretic and serological characterization of Porthetria dispar polyhedron proteinJournal of Invertebrate Pathology, 1976
- A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresisAnalytical Biochemistry, 1967
- BACTERIOLYSIS OF ENTEROBACTERIACEAE IJournal of Bacteriology, 1961
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951