DNA polymerase: evidence for multiple molecular species.

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Abstract
The molecular weights of isolates possessing DNA polymerase activity are in the approximate ratio 4:1:1 implying a tetramer-dimer-monomer relationship which suggests a subunit structure for DNA polymerase. Conformational changes of the polymerase may play a functional role; there are 2 fractions with a molecular weight of 120,000, 1 approximately 4 times as active as the other. The fact that the least active form elutes much faster from G-100 indicates that it is in an extended conformation which may be an inactive state. There is an active fraction of lower molecular weight (78,000) which could have arisen from the 120,000 molecular weight species by cleavage along the long axis of the molecule, as shown by calculations for the dimensions of the equivalent ellipsoids.