Dried gel hybridization in place of Southern hybridization for detection of Listeria monocytogenes DNA fragments

1 June 1991

journal article
Published by Oxford University Press (OUP) in Letters in Applied Microbiology

Vol. 12 (6) , 224-227
https://doi.org/10.1111/j.1472-765x.1991.tb00545.x

Abstract

A simple, sensitive, dried gel DNA hybridization method for detection of Listeria monocytogenes DNA fragments is described. DNA samples were fractionated on an agarose gel. The gel was then denatured in NaOH-NaCl and neutralized in Tris-NaCl. The resulting agarose gel was dried and hybridized with 32P-labelled DNA probe. No transfer to nitrocellulose membranes was used.

Keywords

LISTERIA
DNA FRAGMENTS
MONOCYTOGENES DNA
DRIED GEL
GEL HYBRIDIZATION
HYBRIDIZATION IN PLACE

This publication has 2 references indexed in Scilit:

Introduction of pAM beta 1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids
Infection and Immunity, 1984