Antigen localization in lymphopenic states. I. Localization pattern following chronic thoracic duct drainage.

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  • 1 November 1966
    • journal article
    • Vol. 11  (5) , 467-74
Abstract
Adult rats, depleted of thoracic duct lymph for 5–7 days, were tested for their ability to localize 125I-labelled polymerized flagellin from Salmonella adelaide. Labelled antigen was injected into both hind footpads 6–12 hours after completion of drainage, and the regional nodes were excised 24 hours later. Grain counts on identically exposed autoradiographic sections from regional nodes were used to assess differences in antigen distribution between depleted and nondepleted rats. The uptake of antigen by medullary macrophages was no different in the two groups. However, the uptake of antigen by primary lymphoid follicles was reduced by thoracic duct drainage to levels one-fourth that observed in normal rats.