Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective system
- 1 January 1977
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 18 (3) , 136-148
- https://doi.org/10.1159/000130758
Abstract
A human-mouse hybrid, DUR 4 (Solomon et al., 1976), containing a human X/15 translocation chromosome and also chromosome 5, among other human chromosomes, was used in a double selection system to obtain hybrids of four different types: X/15+ 5+, X/15– 5+, X/15+ 5, and X/15– 5–. Standard positive and negative selection systems were used for the X chromosome, and negative selection for chromosome 5 was done with diphtheria toxin. The assignment of HEXB and the diphtheria toxin sensitivity gene to chromosome 5 was confirmed. HEXA was present only when both the X/15 chromosome and chromosome 5 were present. A “HEXA-like” band segregated with chromosome 15 (or X/15) but independently of chromosome 5. This component, unlike HEXA, does not contain human HEXβ antigen.Keywords
This publication has 2 references indexed in Scilit:
- Characterization of heteropolymeric hexosaminidase A in human X mouse hybrid cells.Proceedings of the National Academy of Sciences, 1976
- Characterization and tissue distribution of N-acetyl hexosaminidase C: suggestive evidence for a separate hexosaminidase locusAnnals of Human Genetics, 1976