Increase in temperature induces the Z to B transition of poly[d(G‐C)] in water‐ethanol solution
- 23 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 173 (1) , 243-246
- https://doi.org/10.1016/0014-5793(84)81055-8
Abstract
An increase in temperature from 20 to 50° C results in the complete transition from the Z to B form of poly(d(G-C)], dissolved in a 55% ethanol-water solution. The transition is fully reversible and displays a slow kinetics. The transition profiles for the free polynucleotide and for that in the presence of ethidium bromide, which is known to stabilize the B form, are obtained by circular dichroism. Based on these data the enthalpy value for the B-Z transition in our conditions is estimated to be ΔH BZ = −0.7 .
Keywords
This publication has 12 references indexed in Scilit:
- Energetics of B-to-Z transition in DNA.Proceedings of the National Academy of Sciences, 1983
- A thermally driven interconversion of B and Z-DNABiochemical and Biophysical Research Communications, 1983
- Antibodies specific for left-handed Z-DNA.Proceedings of the National Academy of Sciences, 1981
- Left-handed DNA in restriction fragments and a recombinant plasmidNature, 1981
- Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).Proceedings of the National Academy of Sciences, 1981
- The transitions between left- and right-handed forms of poly(dG-dC)Nucleic Acids Research, 1981
- Polymorphism of a synthetic DNA in solutionNature, 1976
- Ethidium Bromide as a Cooperative Effector of a DNA StructureProceedings of the National Academy of Sciences, 1972
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972
- Perspectives report: Melting of DNA: Its study and application as a research methodBiopolymers, 1970